Fermentas TAE 50X Buffer ( Tris-acetate-EDTA)

Applications Electrophoresis of nucleic acids in agarose and polyacrylamide gels. Used both as a running buffer and as a gel preparation buffer. Filtered through a 0.22 µ m membrane. Recommended for resolution of RNA and DNA fragments larger than 1500 b( p) , for genomic DNA and for large supercoiled DNA. 1X Composition 40 mM Tris, 20 mM acetic acid, 1 mM EDTA, pH of 50X TAE: 8.4 Usage Recommendations Use fresh 1X TAE both for the gel and for the electrophoresis run. To prepare 1X TAE buffer add 20 ml of 50X TAE buffer to 980 ml of deionized water and mix well. Quality Control The absence of endo-, exodeoxyribonucleases and ribonucleases confirmed by appropriate quality tests.